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For Preparation of donor BMCs and IBM injection of BMCs

The BMCs collect from the femurs and tibias of B6 mice. The IBM injection was carried out as follows. The region from the inguen to the knee joint was shaved of hair with a razor and a 5-mm incision was made on the thigh. The knee was flexed to 90° and the proximal side of the tibia was drawn to the anterior. A 26-gauge needle was inserted into the joint surface of the tibia through the patellar tendon and then inserted into the bone marrow cavity. Using a microsyringe (50 μL; Hamilton, Reno, NV) containing the donor BMCs (3 × 107/30 μL), the donor BMCs were injected from the bone hole into the bone marrow cavity.

For PV injection of BMCs

The BMCs were injected via the PV, as described previously.25 In brief, a midline incision was made on the abdomen to expose the viscera. The donor BMCs (3 × 107/300 μL) were injected via the superior mesenteric vein using a 27-gauge needle.

For Experimental protocols

The onset of autoimmune diseases in MRL/lpr mice was monitored by proteinuria (> 2.5+) and lymphadenopathy. The mice (4-5 months of age) with autoimmune diseases were irradiated in fractionated doses (5.5 Gy × 2 = 11 Gy; 4-hour interval). One day after the irradiation, the mice were transplanted with the whole BMCs (3 × 107) by IBM injections (abbreviated as 5.5 Gy × 2 + IBM). Two groups were prepared as controls. The first group consisted of mice that were irradiated (5.5 Gy × 2) and transplanted with the whole BMCs (3 × 107) via the PV (abbreviated as 5.5 Gy × 2 + PV). The second group of mice was irradiated (5.5 Gy × 2) and transplanted with the whole BMCs (3 × 107) via the tail vein (abbreviated as 5.5 Gy × 2 + IV). Furthermore, recipients that had received reduced irradiation doses (5 Gy × 2, or 4.5 Gy × 2) plus IBM-BMT, PV-BMT, and IV-BMT were also prepared.

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http://www.ncbi.nlm.nih.gov/pubmed/19099639

Just so you know, most transplantations using MSCs do not end up with a significant amount of engraftment.  Most studies that have tracked engraftment show that very few cells survive more than a week (or even 3 days) post-injection (see Gao et al. in my publications).  There are, however, some more recent studies that show MSC engraftment and these generally entail engraftment in regions that have had damage or are inflamed.  See Lin et al. (my publications) for an example of transplantation to an injured site, although this was with a cell line, and Kean et al. for a review of transplantation in general.   The ability of MSCs to home and engraft is not as great as you might expect from reading the literature. 

MSCs are very resilient for transplantation. We implant them on tissue engineering scaffolds using only blood from medullary bone. They remain viable incorporated in the scaffolds on operating tables for often 10 - 15 minutes or longer with no protection or processing before implantation. The real issue is the degree and extent of the immunologic response. Autograft bone marrow transplantation is a well established, documented procedure.