Is there anyone familiar with renal cell carcinoma cell lines, such as 786-O? I am trying to find what kind of cells they are, for example if they are proximal tubule epithelial cells. Also, would the culture conditions RPMI 1640/ 10% fetal bovine serum media be ideal? Any idea or reference would be very appreciated.
Anna Antonina Sobocińska
Hello!
In my lab We use RPMI 1640 with 10% FBS and antibiotics. It works well - 786-O is not demanding cell line and it grows really fast - as well as for an example ACHN cell line. Please be aware of which cell type would you like to work on - clear cell, papillary and so on - sometimes they are with wrong description. If you have any more questions please do not hesitate to ask me. Have a nice day.
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