Has anyone used dextranase to dissolve microcarriers?

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Is there someone who is interested fo a Qualitative Research (by interview) for my thesis " The Role of Arificial Intelligence in Crisis Management' ?

I am struggling, to find researchers, to answer to four questions (simple) for my Thesis. I have a feedback, only by one. Must have a background to Artificial Intelligence. Hopping for great...

13 January 2024 3,913 3 View

Could anyone recommend a cleaning protocol for electrodes that are dipped in algal culture?

I am working on my master's thesis, which is about producing electricity from microalgal cultures. During the experiment, I will have to dip electrodes (graphite rod, carbon cloth, carbon felt,...

09 May 2022 1,287 2 View

Is anyone familiar with 786-O renal cell carcinoma cell characteristics?

Is there anyone familiar with renal cell carcinoma cell lines, such as 786-O? I am trying to find what kind of cells they are, for example if they are proximal tubule epithelial cells. Also, would...

04 June 2014 3,315 3 View

Publishing thesis in a peer-reviewed journal without paying .

Dear collegues, I would like your experience regarding the publication of our research projects. As a postgraduate student (Msc) I am required to publish my research project in a peer reviewed...

01 January 1970 5,052 4 View

Can sulfate dextran be a substrate for dextranase?

Hello every, I am looking for a commercial enzyme that can degrade sulfate dextran. What is known at present is that dextran can be a substrate for dextranase. so I want to know if sulfate...

23 May 2024 1,471 0 View

Can I mix TRITC-labeled dextran and FITC-labeled lectin for one injection instead of having to do two injections of each?

For my protocol, at end-point I have to do tail-vein injections of TRITC-labeled dextran (100 ml of 10 mg/ml) and FITC-labeled lectin (50 mg). However, the protocol I'm following states that they...

01 May 2024 8,090 1 View

How long do you leave endothelial cells to grow on Transwells before performing macromolecular permeability assay?

I want to test the effect on endothelial permeability of certain compounds. I am using Transwell filters (0.4 microns pore size), FITC conjugated dextran (40 kDa) and Human Aortic Endothelial...

20 December 2023 7,641 1 View

How to lower the molecular weight of iron dextran(iron isomaltoside)?

Procedure of making my samples. i Weighed 1 g of dextran with a molecular weight of 1000Da, add 4 mL of water, and dissolve in a 550C water bath. After dissolution of dextran properly i added...

11 October 2023 7,459 0 View

What is the protocol to stain LysoSensor™ Yellow/Blue dextran, 10,000 MW, Anionic, Fixable?

I am seeking assistance and guidance regarding the staining of MDMs with LysoSensor™ Yellow/Blue dextran, 10,000 MW, Anionic, Fixable. Specifically, I am interested in understanding if there is a...

26 September 2023 8,848 1 View

How many neutrophils do people generally get from a neutrophil isolation protocol (using Histopaque/Dextran)?

Trying to calculate how much blood I would need to draw in order to get a sufficient number of neutrophils (from which extracellular vesicles will be obtained).

05 June 2023 5,739 2 View

What is the relationship between PEG/Dextran concentration and mixing ratio and the size and concentration of proteins in the ATPS system?

I want to apply PEG/Dextran system to separate specific-sized molecules from a bio-complex system containing various biomolecular components. I would like to know how Dextran concentration and...

08 December 2022 5,106 0 View

How Dextran sodium sulphate treatment shorten the colon length ??

People use Dextran sodium sulphate to induce colitis, as a observation colon length shorten. What is actual changes happened during treatment resulting in shorter length of colon

08 August 2022 7,058 2 View

Why titration is done to determine the degree of oxidation in a Poly Dextran Aldehyde solution?

While determining the oxidation% of my PDA solution in my hydrogel project, I am suddenly curious why we do this, this way.

22 February 2022 3,859 3 View

Dextran experiment - Some cells permeabilizing and others not?

Hi peers, I have been optimizing a dextran experiment in my laboratory by which I want to permeabilize the plasma membrane but not the nuclear one. That is, because I want to assess the transport...

03 February 2022 3,356 1 View