Has anyone used dextranase to dissolve microcarriers?

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Publishing thesis in a peer-reviewed journal without paying .

Dear collegues, I would like your experience regarding the publication of our research projects. As a postgraduate student (Msc) I am required to publish my research project in a peer reviewed...

01 January 1970 4,994 4 View

Why HEK293 transfected with LAMP-1GFP do not load dextran?

Hi every one I have made a cell line of HEK293 transfected LAPM-1GFP and after seeding the cells for 24h, I try to load dextran on the dish but after imaging, the cells with GFP+ have loaded less...

14 December 2020 1,801 2 View

Does anybody know the difference of the effect of the dextran coated charcoal stripped serum (DCSS) containing medium and DCSS containg medium?

Hi everybody, I have cultivated the C4-2 cell line in double charcoal stripped serum containing medium. A lot of the cells do not attach to the bottom of the flask. If someone has experience...

24 August 2020 9,634 2 View

What is a suitable purification method for big dextrans (> 2000 kDa) beside size exclusion chromatography?

Is capillary electrophresis or ion exchange chromatography a convenient method when charges are introduced into the dextran?

06 February 2020 7,946 6 View

Could someone suggests which MW of FITC-Dextran is used for chemotaxis of bacteria?

Could someone suggests which MW of FITC-Dextran is used for chemotaxis of bacteria? Also, if I make a FITC-dextran solution in PBS then how long can we store this solution at 4 degree Celsius?

31 December 2019 3,589 1 View

How do I boost the signal of an Alexa594-conjugated dextran?

I am working on a project tracing projections using an Alexa594-conjugated dextran. I am interested in very fine processes which I'm having difficulty distinguishing from my autofluorescent...

16 December 2019 8,003 3 View

How do I boost the signal of an Alexa594-conjugated dextran?

I am working on a project tracing projections using an Alexa594-conjugated dextran. I am interested in very fine processes which I'm having difficulty distinguishing from my autofluorescent...

11 December 2019 6,379 3 View

What are the best and possible methods fro separation of glucose molecules ( monomere & dimer) from a polysaccharide mixture?

Her, I have a Dextran ( mw : 15, 000) , a bioplymer polysaccahride, and i m perfoming the hydrolsysis part by acid treatment & higher temp. to get desired molecular weight of polysaccaride (...

07 August 2019 4,415 4 View

I'm working on a kit that contains small amount of peptide (10ug) with large amount of dextran. What is the best way to purify the peptide?

I need this to quantify the peptide by HPLC methods (analytical assay). The peptide is 900 Dalton. The Dextran is 70000 Dalton.

17 April 2019 3,206 6 View

I'm working on a kit that contains small amount of peptide (10ug) with large amount of dextran. What is the best way to purify the peptide?

I need this to quantify the peptide by HPLC methods (analytical assay). The peptide is 900 Dalton. The Dextran is 70000 Dalton.

03 April 2019 1,979 7 View

Are there any easily preparable/ cheap colored gel filtration standards?

Are there any easily preparable/ cheap colored gel filtration standards? I have blue dextran

10 November 2018 1,517 1 View