Is it recommended for frozen human stool samples? I need the bacterial DNA out of these samples. I tried the stool DNA isolation kit of QIAGEN, but the i-genomic is cheaper and planning to continue with it.
Dear Suheir
Dr. P.P.Nair and his team have been working for the past 30 years on isolation ,characterization and molecular pathology. Please follow his protocols.
The procedures allow clean separation of bacteria from the exfoliated epithelial cells called colonocytes
http://cebp.aacrjournals.org/content/5/11/913.long
Examples
http://www.ncbi.nlm.nih.gov/pubmed/8922300
Gastroenterology. 1998 Jun;114(6):1333-5.
Noninvasive detection of colorectal cancer by molecular tools: coming of age.
Dutta SK, Nair PP.
PMID: 9618652 [PubMed - indexed for MEDLINE]
check out www.thermofisher.com/microbiome for complete microbiome workflow solutions
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