Suheir Nassar

4 Questions 10 Answers 0 Followers

Questions related from Suheir Nassar

How do you deal with Bacterial DNA degradation and get a PCR product?

DNA was extracted from three anaerobic Gram positive bacteria, (C.difficile,B.bifidium and B.lactis) using a QIAGEN kit. PCR was performed for 16S rDNA segment specific for each one. Only B.lactis...

16 September 2014 8,664 2 View

Can anyone help with reference gene selection for qPCR gene expression analysis?

I am going to test the gene expression of IL-33 , Hinf I and other immune response related genes in blood. I am confused about the reference gene that I should select and order primers for along...

04 September 2014 7,090 6 View

Is anyone experienced in the use of the i-genomic Stool DNA Extraction Mini Kit from INTRON biotechnology?

Is it recommended for frozen human stool samples? I need the bacterial DNA out of these samples. I tried the stool DNA isolation kit of QIAGEN, but the i-genomic is cheaper and planning to...

08 May 2014 2,021 2 View

Which TRI reagent is preferred for use in RNA isolation?

I'd like to ask if there is difference in TRI reagent supplied by several companies: Sigma, Peqlab, invitrogen...etc. Is there any one preferred for use for RNA isolation?

17 April 2014 573 10 View