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Questions related from Suheir Nassar
Would broth by its contents act later as PCR inhibitor after DNA isolation from bacterial cells pelleted from broth.? Concerning DNA isolation from anaerobic bacteria..is there a special...
22 September 2014 6,020 13 View
DNA was extracted from three anaerobic Gram positive bacteria, (C.difficile,B.bifidium and B.lactis) using a QIAGEN kit. PCR was performed for 16S rDNA segment specific for each one. Only B.lactis...
16 September 2014 8,708 2 View
I am going to test the gene expression of IL-33 , Hinf I and other immune response related genes in blood. I am confused about the reference gene that I should select and order primers for along...
04 September 2014 7,146 6 View
Is it recommended for frozen human stool samples? I need the bacterial DNA out of these samples. I tried the stool DNA isolation kit of QIAGEN, but the i-genomic is cheaper and planning to...
08 May 2014 2,065 2 View
I'd like to ask if there is difference in TRI reagent supplied by several companies: Sigma, Peqlab, invitrogen...etc. Is there any one preferred for use for RNA isolation?
17 April 2014 619 10 View