Suheir Nassar

5 Questions 15 Answers 0 Followers

Questions related from Suheir Nassar

Is it preferred to isolate bacterial DNA from bacterial cells cultured in broth or on agar?

Would broth by its contents act later as PCR inhibitor after DNA isolation from bacterial cells pelleted from broth.? Concerning DNA isolation from anaerobic bacteria..is there a special...

22 September 2014 6,023 13 View

How do you deal with Bacterial DNA degradation and get a PCR product?

DNA was extracted from three anaerobic Gram positive bacteria, (C.difficile,B.bifidium and B.lactis) using a QIAGEN kit. PCR was performed for 16S rDNA segment specific for each one. Only B.lactis...

16 September 2014 8,717 2 View

Can anyone help with reference gene selection for qPCR gene expression analysis?

I am going to test the gene expression of IL-33 , Hinf I and other immune response related genes in blood. I am confused about the reference gene that I should select and order primers for along...

04 September 2014 7,151 6 View

Is anyone experienced in the use of the i-genomic Stool DNA Extraction Mini Kit from INTRON biotechnology?

Is it recommended for frozen human stool samples? I need the bacterial DNA out of these samples. I tried the stool DNA isolation kit of QIAGEN, but the i-genomic is cheaper and planning to...

08 May 2014 2,068 2 View

Which TRI reagent is preferred for use in RNA isolation?

I'd like to ask if there is difference in TRI reagent supplied by several companies: Sigma, Peqlab, invitrogen...etc. Is there any one preferred for use for RNA isolation?

17 April 2014 625 10 View