Is alpha tubulin a good loading control for western blot of purified membranes from mouse tissues?

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How do I determine the mg of ascorbic acid equivalent to 1.0 mL of standard indophenol solution?

Hello everyone. Could you help me with the following problem. I am going to determine ascorbic acid in capsules using an AOAC method. The method gives the following equation: mg ascorbic...

24 June 2024 789 1 View

Metodology for dashboard, please?

Please scientific articles on methodologies to create dashboards in R shiny and PowerBI

27 May 2024 387 1 View

What are the UV ranges and temperatures mostly effect on microplastic degradation?

For a study about the effect of UV and temperature on plastic degradation and to compare the changes between different UV and temperature levels by using fresh plastic pellets. What would be the...

22 March 2024 4,067 1 View

Do you have seed trait data for tropical species?

We would like to invite you to build an inclusive, open-access database on seed traits for tropical species. If you have morphological, biophysical, or germination data for tropical species,...

07 March 2024 2,897 4 View

Você trabalha com traços funcionais de plantas de Cerrado?

Nós gostaríamos de te convidar para participar e contribuir com com o Cerrado Plant Traits: uma rede para a construção de um banco de dados de traços funcionais de plantas. Se você tem dados...

27 February 2024 2,142 2 View

DDS and Pharmaceutical industry. Conferences opinions?

Hello everyone! I am considering the possibility of assisting Congress or Conferences on Drug Delivery Systems or Pharmaceutical Formulation. However, I do not have much experience in this...

30 January 2024 7,440 0 View

A good anti-GR antibody for western blot in plants?

Hello, I would like to perform a western blot to detect a protein fused to GR (Glucocorticoid Receptor) and expressed from a 35S promoter in transgenic apple plants. I have never used anti-GR...

17 January 2024 7,806 0 View

Independant T-test Vs Paired- T Test results?

What if the Paired T-Test results are significant, but Independent T Test results are not significant? (Done in the same sample) Ex: Paired T-Tests done in the Experimental groups are...

12 January 2024 3,288 4 View

¿Te interesaría hacer divulgación en YouTube? Interested in doing outreach on YouTube?

Estimados Colegas Disculpen las molestias. Estoy intentando hacer divulgación científica por medio de un canal de YouTube –se llama Datos y Ciencias Sociales, el link es...

20 December 2023 3,340 1 View

Abaqus error rfm_ArrayRepr::rfm_ArrayRepr when reading .ODB file?

Dear colleagues, I have a python script to read results from ODBs in Abaqus. More specifically, I am extracting the equivalent plastic strain PEEQ from different regions (sets previously...

28 November 2023 3,311 4 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View