Western Blot Troubleshooting - help please?

16 March 2024 1 7K Report

Hi all, need a little help with this western blot please.

Protein band seems pretty spread out and this isn't the first time it's happened, thinking something must be wrong with the gel or protein degradation?

Any help appreciated!!

Adam B Shapiro

Is the protein glycosylated? That would tend to cause the band to broaden. To test for N-linked glycosylation, treat the sample with PNGase F.

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