I want to convert, for example, Adenine into Guanine or vice versa. Is there any specific chemical agent/enzyme available that will assist this type of conversion?
Sir First you should convert adenine -C present in between nitrogen to C-Br then convert into NH2 means guanine .
Intra-conversion of Purine bases or pyrimidine bases are possible through different reactions such as amination, deamination, oxidation,reduction halogenation, dehydrohalogenation , enzymatic reactions etc.
Thank you, everyone. Consider a particular situation. For example, I want to convert all G into A of 30 nucleotides long dsDNA/ssDNA without rest of the nucleobases unaffected. Could you help me a bit deeper? Zakia Sultana Nishat
Madhukar Baburao Deshmukh Naimish Kumar Verma
The conversion of the amino to the oxo group takes place immediately by adding sodium nitrite to the solution of the amino derivative in conc. sulfuric acid with stirring at RT. The reverse process is more complicated, in the first stage of the conversion of oxo to chloro (POCl3 / PCl5 e.g.), Then the substitution of chlorine with ammonia.
https://www.the-scientist.com/daily-news/base-editing-now-able-to-convert-adenine-thymine-to-guanine-cytosine-30706?fbclid=IwAR07hb75HlTtxQx2krpKuJGWS5ivqLBNqWqwgoHMlTHgwiWSuW516esNyDw
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