Rabbee G. Mahmudunnabi

11 Questions 14 Answers 0 Followers

Questions related from Rabbee G. Mahmudunnabi

Is there any software available for designing primers which can take multiple sequence input as fasta format?

I would like to design primers for 18S rRNA gene of Cryptosporidium spp. Usually, I use NCBI to design primers. Then I check the primers with multiple sequences alignment in MEGA or design the...

10 January 2024 493 5 View

How to design qPCR primer for mature microRNA?

I am going to buy GoTaq® 2-Step RT-qPCR System(Promega). This kit uses oligo(dT)15 primer for first-strand cDNA synthesis. I have to design primers for qPCR for 4 mature microRNAs(miR-21, miR-155,...

10 November 2020 9,387 3 View

Is there any relationship between chronoamperometric current response and surface resistance of electrode?

I am using hydrazine as an electrocatalyst to reduce hydrogen peroxide. And took CA to measure the current. Meanwhile, surface resistance was measured through impedance spectroscopy.

07 May 2020 3,717 0 View

How to make RNase free PBS buffer?

I know that DEPC treatment can make water RNase free. If I use DEPC-treated water for making PBS, I think, at the time of checking the pH the solution may be contaminated with RNase. Is it...

23 July 2019 7,815 8 View

How to detach a cell/exosome from the antibody without disrupting other proteins of cell/exosome?

Assume that, I am using magnetic bead that is modified with CD9 antibody and captured the exosome. Now I want to collect only specifically captured exosome leaving the magnetic bead/antibody complex.

10 March 2019 2,523 1 View

What is the reason for getting different peaks in Dynamic Light Scattering(DLS) of exosome in different measurements of same sample?

I prepared the Exosome sample through urltacentrifugation. In the final stage of ultracentrifugation, Exosome sample was resuspended on 0.1M PBS of PH 7.4. I got around 450nm peak In the first...

19 February 2019 320 13 View

Can someone help with a "Target.pdbqt" preparation problem?

During preparation of .pdbqt file I had a warning like that : Total kollman charge added = -166.952. What's that? explain please.

27 November 2014 8,533 5 View

Who can please explain "marginal stability of protein" and "conformational entropy"?

I'm studying negative sense RNA virus virus fusion with host membranes. But I don't have much understanding about protein folding and unfolding, conformational stability. I need detail about this...

14 November 2014 8,912 4 View

Why does substitution of Glycine with proline can not affect the c-ras gene?

The ras genes appear to be finely alanced at the edge of oncogenesis. Almost any mutation at either position 12 or 61 can convert a c-ras proto-oncogene into an active oncogene. All C-ras genes...

29 September 2014 2,348 1 View

What is ''stringent cell line''?

I was studying a paper titled ''Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.'' There I found the term cell line isolate...

01 January 1970 1,519 3 View

Intra-conversion of Purine bases or pyrimidine bases.

I want to convert, for example, Adenine into Guanine or vice versa. Is there any specific chemical agent/enzyme available that will assist this type of conversion?

01 January 1970 3,155 6 View