Interaction between Horses and Humans?

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Water Consumption in Plastic Recycling: Is it Higher Than for Virgin Plastics?

Hello, dear colleagues, I would like your help in finding a reference that provides an average water savings due to plastic recycling compared to the consumption of virgin materials, preferably...

29 July 2024 4,630 0 View

A variable moderating multiple relation - how can I test hypotheses using amos?

How can I analyse the relationship between five independent variables and one dependent variable, with a moderating variable influencing each of these five relationships? How can I test hypotheses...

03 June 2024 6,221 1 View

Any experience in fibroblast centrifugation?

We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...

28 May 2024 3,529 1 View

Fibroblast trypsinization in Hanks medium with Ca and Mg?

We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....

28 May 2024 6,052 2 View

Are the calibration curves of NO radical scavenging assay asymtotes?

Hello. I am here looking for help with the NO radical scavenging assay. I was working on setting up the assay with sodium nitroprusside and the Griess reagent as they do in most works. However, I...

27 May 2024 5,013 1 View

Inconsistente results with cell line LX-2, any advice?

Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...

21 May 2024 2,171 0 View

Will ultracentrifugation be replaced as the most widely used method for isolating extracellular vesicles?

Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.

16 May 2024 9,432 1 View

¿What control can I use to inhibit the proinflammatory effect of LPS in macrophagues?

I want to evaluate the polarization to M2 phenotype in Raw Cells, I co-cultured the cell with LPS and my drug to see the effect of the drug to inhibit the proinflammatory effect of LPS, but I have...

05 May 2024 8,525 0 View

Scientific support for research in Dentistry University of Los Andes Venezuela?

Sirs. ResearchGate Receive a cordial and respectful greeting. For academic purposes only, we request collaboratively the following articles for one of our research professors, who is preparing...

05 May 2024 9,459 9 View

How can we prevent small peptides (1000 Da) from eluting out of the gel during staining in a peptide gel (tricine-SDS-PAGE, Shagger)?

How can we prevent small peptides (1000 Da) from eluting out of the gel during staining in a peptide gel (tricine-SDS-PAGE, Shagger)? There are different types of fixing solutions, but I'm not...

28 April 2024 1,522 0 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

What is the current status of augmented learning in robotic surgery?

I would like to perform a literature review at this time on augmented learning and learning augmented algorithms to enhance performance-guided surgery

06 July 2024 246 1 View

Has anyone used the new E1 Clip tip digital pipettes?

We just purchased a multichannel and a single channel E1 Clip tip pipettes. In our first attempt to use them for an experiment they are not dispensing accurately? The rep told me that...

01 July 2024 1,983 0 View

What are the primary indications for choosing neuraxial anesthesia over general anesthesia in surgical procedures?

The Evolution and Application of Spinal Anesthesia: Techniqu... What are the primary indications for choosing neuraxial anesthesia over general anesthesia in surgical procedures?

27 June 2024 5,763 1 View

Did you ASK AI (https://talkai.info/tr/chat/) the same question?

What food do intestinal bacteria like most?ChatGPTIntestinal bacteria love fibrous foods that are prebiotic. These foods include vegetables (such as broccoli, cabbage, and brussels sprouts),...

22 June 2024 4,567 0 View

How can we train multi-modal CLIP architecture to generate images using Prompt ?

Can we even make changes to CLIP Model architecture such that it can be used as an image generator from prompts ?

16 June 2024 320 0 View

Do plants produce hormones that have the same influence properties of animal hormones?

I know that all animals and insects produces hormones. Do plants produce hormones that can have an equivalent effect on an animal like animal hormones?

13 June 2024 3,860 1 View

Will Pluset (FSH and LH) aliquots kept in -20°C work after thawing?

Hi. I would like to know your oppinions on freezing superstimulatory hormones (namely Pluset, Calier) and keeping them in -20°C. In was using Stimufol in our lab and it was working fine when...

30 May 2024 9,497 0 View

If you have a missing trapezoid in total AUC for salivary cortisol data, is this representative of the cortisol changes to estimate sleep changes?

Participants completed 6 saliva samples throughout the day to capture changes in cortisol and melatonin from waking until just before sleep. To achieve total AUC, 5 trapezoids between samples 1-6...

27 May 2024 6,842 0 View

In vitro root growth in oil palm (hardy plant)?

Im working on oil palm tissue culture we are using MS media along with IBA and NAA hormones for platlets but the response was not that good can anyone suggest if any changes needed in media and...

15 May 2024 4,607 1 View