Inquiry Regarding Nickel Sepharose Resin Color Change and CIP Protocols?

16 December 2024 1 8K Report

Dear Colleagues,

I am encountering an issue with my Nickel Sepharose resin, as it becomes colored after a run. I would like to seek your advice on potential solutions to this problem and would also appreciate any guidance on the regular cleaning-in-place (CIP) procedures recommended for Nickel Sepharose resin between runs.

Thank you for your assistance.

David Liang

Regeneration

To effectively restore the resin’s binding capacity and remove residual contaminants:

Strip Ni²⁺ from the resin using 0.1 M EDTA, ensuring complete removal of metal ions.

Regenerate the resin with 0.5 M NaCl to further clean and prepare it for reuse.

Storage

After regeneration, store the resin under one of the following conditions to prevent microbial growth:

In deionized water (DI H₂O) with 0.03% sodium azide, which acts as a preservative.
In 20% ethanol, providing an alternative antimicrobial storage solution.

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