12 March 2021 0 4K Report

I have been having an issue with getting reproducible data from my western blot analysis. I apply ECL, develop the films, and obtain bands that look one way (usually a decrease in intensity as I move right). When I wash the membrane and go through the development steps again, I sometimes get different band intensities. As a result of this, I am unable to know if the results I am seeing are real or artifacts. Any advise?

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