Could someone please provide me with a reliable differentiation protocol for IMR-32 NB cells?

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Alexandr Chernov

Dear Shehab!

Could You look at this article and protocol, please:

In Vitro Differentiation of Human Neuroblastoma Cells Caused by Vasoactive Intestinal Peptide

https://cancerres.aacrjournals.org/content/canres/50/16/5177.full.pdf

Fig. 5. Pseudoganglion formation and neurite extension in IMR-32 neuroblastoma cells treated with (ft) 1.0 mkM VIP

Bashier Ibrahim Osman

https://www.frontiersin.org/articles/10.3389/fnmol.2017.00310/full