Has anyone performed or come across someone that performed organotypic brain slice cultures on Guinea Pig brains?
Hi all, I intend to create a point mutation in the genome of C2C12 cells but am having difficulty deciding what ratio's of gRNA plasmids, Cas9n plasmid, and ssODN repair template. I am...
29 May 2021 5,171 0 View
I have been having an issue with getting reproducible data from my western blot analysis. I apply ECL, develop the films, and obtain bands that look one way (usually a decrease in intensity as I...
11 March 2021 4,297 0 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 4,128 3 View
Treatment with 10uM Retinoic acid seems to result in vacuolization and cell death
20 January 2021 4,251 2 View
01 January 1970 3,276 2 View
01 January 1970 9,100 0 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...
28 July 2024 7,522 0 View
I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?
26 July 2024 4,741 1 View
Hi, I am working on a head FE model and want to try different material types on brain. I read some papers that use Mooney-Rivlin rubber model and wondering if i can add viscosity into it. Is it...
24 July 2024 5,908 0 View
Hi everyone, I experienced some moving particles in my mouse hippocampal slices. The media looks clear. Any idea what these moving particles might be? Thanks you!
22 July 2024 3,734 0 View