Shehab Eid

7 Questions 1 Answers 0 Followers

Questions related from Shehab Eid

CRISPR-Cas9n HDR in C2C12 Cells?

Hi all, I intend to create a point mutation in the genome of C2C12 cells but am having difficulty deciding what ratio's of gRNA plasmids, Cas9n plasmid, and ssODN repair template. I am...

30 May 2021 5,130 0 View

Inonsistency in Western Blot Film Band patterns?

I have been having an issue with getting reproducible data from my western blot analysis. I apply ECL, develop the films, and obtain bands that look one way (usually a decrease in intensity as I...

12 March 2021 4,259 0 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

02 March 2021 3,960 3 View

Could someone please provide me with a reliable differentiation protocol for IMR-32 NB cells?

Treatment with 10uM Retinoic acid seems to result in vacuolization and cell death

21 January 2021 4,173 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 January 1970 3,211 2 View

Guinea Pig Organotypic Brain Slices

Has anyone performed or come across someone that performed organotypic brain slice cultures on Guinea Pig brains?

01 January 1970 9,529 0 View

Western Blot transfer issues (uneven transfer)?

I have been having an issue with getting reproducible data from my western blot analysis. I apply ECL, develop the films, and obtain bands that look one way (usually a decrease in intensity as I...

01 January 1970 9,069 0 View