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Hi all, I intend to create a point mutation in the genome of C2C12 cells but am having difficulty deciding what ratio's of gRNA plasmids, Cas9n plasmid, and ssODN repair template. I am...
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Has anyone performed or come across someone that performed organotypic brain slice cultures on Guinea Pig brains?
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Hello, I have consistently had issues with uneven western blot transfers at the lower MW regions (as seen in the attached picture). The transfer system is the Thermo Mini Blot Module Wet transfer...
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