I want to inject nude mice with THP1 macrophages. has anyone tried injecting differentiated THP1 into mice? Do you let them differentiate in plates? and if so how do you detach them with ought killing?
Hello, Elena!
You look at this article, please:
Article Microvesicle-mediated RNA Molecule Delivery System Using Mon...
Cell culture. Cells of human monocytic cell line THP-1 were grown in RPMI-1640 medium supplemented with 5% (vol/vol) heat-inactivated fetal bovine serum (Sigma, St Louis, MO) and 2 mmol/l L-glutamine under an atmosphere of 95% air and 5% CO2 at 37 °C.
Human tumor xenograft model and administration of miR-143BP-transfected THP-1 macrophages. Animal experimental protocols were approved by our University's Committee for Ethics in Animal Experimentation, and animal experiments were conducted in accordance with the guidelines for Animal Experiments of Gifu University. Human colon cancer DLD-1 cells were inoculated at 2 × 106 cells/100 µl per site into the backs of 5-week-old athymic nude mice. The tumor-engrafted mice were sorted into five mice per group. A suspension of miR-143BP-transfected THP-1 macrophages prepared as described in preparation of shed-MV fraction section, was injected intravenously once a day for 2 days.
Thank you for your replay. How did you detach cells from plate though? did you use trypsin?
Hi, you can use PMA to differentiate them. Furthermore, you can detach cells using 0.5% lidocaine + 5 mM EDTA + 30 mM NaN3 in PBS at ph 7 for 15-20 incubation at 37 oC.