Inhibit mechanism of reserpine - any thoughts?

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Why my TiO2 2p1/2 peak higher and wider than 2p3/2 peak(XPS)?

I've never seen these kind of peaks before,what reason can cause it,I'm really confused.

13 November 2023 7,297 6 View

How to furtherly promote the quality of protein-DNA complex crystals?

Hi all, I set up the crystallization of protein-DNA complex for initial screen and found one crystal cluster appeared in a condition (0.1M HEPES pH7.0, 0.2CaCl2·2H2O, 45% MPD). Afterwards I added...

04 May 2023 5,110 1 View

How to furtherly promote the quality of protein-DNA complex crystals?

I set up the crystallization of protein-DNA complex for initial screen and found the condition to optimize (0.1M HEPES pH6.5, 0.2MgCl2, 25% PEG3350). After rounds of optimization, the quality of...

13 April 2023 5,286 0 View

What code can i use to simulate the weakly magnetized plasma?

I'm studying the ion-ion plasma thruster, I want to simulate the magnetic filter in it. The thruster I want to study is similar to that designed by St ́ephane Mazouffrea and Denis Renaud which has...

09 April 2023 5,524 0 View

How to avoid soluble aggregate during protein purification?

Hi all, Recently I am working on protein purification. 1. affinity column(GST) 2. Add PSP enzyme to remove GST-tag(O/N) 3. GST-tag off 4. gel filtration However, the protein tends to form large...

07 November 2022 9,442 2 View

Will excessive template affect the specificity of PCR products?

I am amplifying a target fragment recently. I have done a gradient to determine the annealing temperature, but there are still many specific bands. May I ask you if the amount of template will...

29 June 2021 5,601 2 View

What is the optimal SDS concentration in ChIP assay?

Dear all, I meet a big trouble in ChIP assay. I prepared the cell lysis buffer/shearing buffer containing 1%SDS which helps sonication well. However, 1%SDS damages the epitope of target protein...

24 June 2021 3,763 1 View

Is there a specific PKA activator?

I found 3 drugs that can be used as PKA activator: forskolin, 8-Bromo-cAMP, 6-Bnz-cAMP. Forskolin activates cAMP and can activate PKA. The latter two are cAMP analogs that can activate PKA. I'm...

01 June 2021 3,469 1 View

Is it better to use picrotoxin or gabazine to block IPSC when studying EPSC?

I think both are used mainly for blocking GABAA receptors. Since gabazine is more selelctive for GABAA receptors, is it better to use for studying EPSC? Is it enough to block IPSC by only blocking...

06 March 2021 1,722 6 View

What type of research should do when obtained zebrafish genes knock-out mutant?

I have got zebrafish knock-out mutants with crisper-cas9 method, I only observed mutant possessed obvious phenotype and did genes qRT-PCR analysis. however, I didn't know how to design next step...

27 June 2020 3,465 0 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How can i study the carboplatin using spectroscopic technique, viz Raman and FTIR spectroscopy?

I'm working on carboplatin which i bought from local pharmacy. It is a Carboplatin injection IP 150mg/15ml which is sterile aqueous solution for intravenous use. Unfortunately, I cannot able to...

04 August 2024 454 3 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View