Laiyin Nie

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Questions related from Laiyin Nie

Inhibit mechanism of reserpine - any thoughts?

I am now working with a H+ dependent multidrug transporter, and I know from several papers that reserpine could be used as an inhibitor. So I tried to use this as an inhibitor, and it worked....

25 June 2014 7,261 3 View

Why does the membrane protein aggregate when purified in larger quantities?

I am now working with a membrane protein, which is a 50KDa transporter with 12 trans-membrane-helixes. The protein is produced heterologously in E.coli with a C-terminal His-tag. I purified the...

19 March 2014 4,622 13 View

One peak in Gel Filtration profile but many bands on SDS gel?

I am working with a membrane protein which has 8 transmembrane segments and a soluble domain at C-terminal. I expressed it in E.coli top10 cells, and tagged it with 10 His in C terminal. I have...

05 September 2013 1,732 21 View