We dabbled a bid with growing papaya cuttings papaya. We looked at cutting size, different mediums , water regimes and commercial root stimulants.
We find it difficult to keep the temperature below 35C and the humidity high. We are using heating cables to keep the temperature higher than 20C (+-25) at the root zone.
We are using clean, filtered water and reverse osmosis.
Our biggest problem is rooting. Few cuttings will root in within 21-days and others are still alive with good callus growth but no rooting. Will tissue culture techniques (chemistry) help to solve the problem?
good day ,this link help you
https://www.ncbi.nlm.nih.gov/pubmed/924458
Dear Aart Louw
Pls. find the attached files and the references, good luck
Hoping this will be helpful to your project
Regards
Prof. Houda Kawas
https://www.fspublishers.org/published_papers/4988_..pdf
https://ejournal.sinica.edu.tw/bbas/content/1992/4/bot334-10.pdf
http://link.springer.com/article/10.1023%2FA%3A1006475901439?LI=true
http://link.springer.com/article/10.1007/BF02627852
http://link.springer.com/article/10.1007%2FBF00269557?LI=true
http://cpi.kagoshima-u.ac.jp/publications/southpacificstudies/sps/sps28-2/SouthPacificStudies28(2)pp87-95.pdf
Thank you Prof. Houda. I am staying clear of tissue culture mediums as I am not interested in making thousands of plants but scores of ten. I am tempting to immerse the cutting first in a sucrose solution for 24-hours before transferring the cuttings to the medium. Composted bark medium and coarse sand are doing equally well.
Also
Its all about hormones ,
indole-3-butyric acid (IBA)
Hoping this will be helpful to your project
http://link.springer.com/article/10.1007%2FBF01997591?LI=true
https://www.jstage.jst.go.jp/article/ecb1963/36/2/36_2_115/_article
https://www.researchgate.net/post/Induce_root_formation_from_papaya_shoots
http://www.ishs.org/ishs-article/812_54
https://www.ndsu.edu/pubweb/chiwonlee/plsc368/lecture/cpt10/cpt10cuttingbio.htm
Article Better Rooting Procedure to Enhance Survival Rate of Field G...
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Hi Aart
Some references in attachment 1
https://ejournal.sinica.edu.tw/bbas/content/1992/4/bot334-10.pdf
4
http://cpi.kagoshima-u.ac.jp/publications/southpacificstudies/sps/sps28-2/SouthPacificStudies28(2)pp87-95.pdf
5
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.546.5971&rep=rep1&type=pdf
11
http://www.cibtech.org/J-Plant-Sciences/PUBLICATIONS/2013/Vol-2-No-1/05-014...Sanjeev...InVitro...Papaya...24-33.pdf
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