Did you just dissolve it in e.g. PBS and formed a stock solution?
Article Reevaluation of bioactivity and antioxidant activity of Myrt...
I want to know more about Uranium ore deposits in world.
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I want to know more about diamond ore deposits in world.
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We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
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I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
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I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
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How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
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I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
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How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
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Hello, I'm looking to dissolve soy lecithin in order to create a liposome. I've tried using various solvents like pure ethanol, methanol, and a mixture of ethanol and methanol but they didn't...
02 August 2024 6,980 4 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
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I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
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I have an issue with SEM imaging of liposomes. When measured by DLS, they appear to be around 120 nm, but when using SEM with simple air drying, they appear much larger, around one micrometer.
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How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?
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Hi All I am looking to get your opinions, I have perfomed liposome mediated DNA transfer into bacteria, the DNA is a plasmid with msfGFP and when I induce the plasmid I see the fluorescence, and...
16 July 2024 4,698 4 View
Hello everyone, I am trying to encapsulate an essential oil in the liposomes using the heating method and encountering some issues. I am following the protocol described below, but my solution...
10 July 2024 4,083 3 View
I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...
01 July 2024 7,007 1 View