I have made a complex of Chitosan and Gum acacia and want to calculate the swelling % in acidic and basic buffers. so I want to know the exact pH in which in should do the experiment
The isoelectric point (IEP) of chitosan can be expected within 7―9, while that of gum acacia should occur by 1―3. Within both IEP ranges, these molecules are oppositely charged, so that they tend to form mutual electrostatic complexes, while dissolved. Coacervation can occur by about the 3―6 pH range. Swelling should be maximized at some point within this same range.