How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

Is there a comprehensive database for Virus Real-Time Primers and Probes ?

Dear All I am looking for a database for tested Real Time PCR primers and probe sequences for the following viruses for diagnostic purposes. Does the USAID PREDICT project or the Global Virome...

01 February 2021 8,920 1 View

I am desigining UWB antenna with ecllipse shaped patch on HFSS.Why gain is coming negative ?

I am attaching my design and result of Gains.S11 parameter is less than -10dB from 3.1 to 9.8 Ghz.Z11 magnitude also lies between 40 -60 ohm for this range .Still gain is negative. Pls help

29 January 2021 4,334 3 View

What are charge states -/0 and 0/+ of Carbon at Nitrogen site?

What is differentiate between these two states? And what's the reason for formation of these charge states?

26 January 2021 6,937 3 View

What causes defect peak broadening?

Say we observe yellow and blue luminescence in GaN, and these defect peaks are always broader than the BE peak. What factors contribute to defect peak broadening? Please help me list out such...

25 January 2021 1,733 3 View

What is the ACTUAL COST OF A PUBLICATION??

Hi. It's 2021 January. Recently, I got to know that Elsevier has converted around 160 journals into some "Transformative Journals" and follow the recommendations under "Plan S"...

19 January 2021 9,985 2 View

In microbiology to do plating is there any need to sterile the DI water also?

In microbiology to do plating, is there any need to sterile the DI water also?

22 December 2020 3,288 1 View

What is the method to prepare input spreadsheets (for nodes & edges tables) from Scopus database, for conducting different network analyses in Gephi?

I am planning to compile a systematic literature review. I have downloaded .csv file of a collection of papers and their respective bibliometric information (authors' names and affiliations, year...

10 November 2020 8,455 3 View

Correct statistical data analysis?

Hi there, I do not have a solid understanding of the statistics - I am seeking advice regarding the correct data analysis. I am helping my colleague to analyze the oxidative stability of oil....

18 October 2020 1,355 6 View

Do we need to take IRB approval for quantitative research paper?

Is IRB Approval necessary for writing a research paper on marketing based on quantitative research?

11 October 2020 3,618 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View