How to prepare RNA loading dye without xylene cynol?

Arisha Arora @Arisha-Arora

27 February 2023 0 9K Report

I need to prepare a dye to mix with my RNA sample before running on gel.

The protocols obtained online contain xylene cyanol as a constituent. Is there a recipe without xylene cyanol?

Please help. Thanks in advance.

Badges
Science topic

Similar topics
Microbiology
Assays

More Arisha Arora's questions See All

What is the difference between Caputo fractional derivative and conformable fractional derivative?

While working on fractional order derivatives I found the behavior of conformable derivative is different from Caputo fractional derivative. Therefore, I want to know the difference between these...

08 July 2024 4,057 1 View

How to position legends below the graph (outside) in Mathematica?

Hello All, I want legends to be positioned below the graph (outside), but in two or three rows accordingly, such that they do not extend beyond the vertical boundaries of the graph. Can anyone...

23 April 2024 7,447 1 View

Can Ostwald viscometer be helpful in measuring viscosities of Non-Newtonian fluids?

I want to check the viscosity of the non-Newtonian fluid using an Ostwald viscometer.

14 January 2024 2,226 6 View

Wrong or mistaken profile?

I had been facing the issue that my name is considered for the publications where neither I am the author nor the co author there is some mistake kindly help to resolve the issue

03 November 2023 9,890 2 View

I am facing difficulties in opening a time series recording file in ImageJ. an error message with insufficient memory comes up. Any solutions?

The available memory keeps changing even if no file is open. The files that I want to open are large files of around 7-10 GB

22 July 2023 8,228 1 View

On the Google search page, when can we expect to see a "Search with AI" button between the default "Google Search" and "I'm Feeling Lucky" buttons?

On the Google search page, when can we expect to see a "Search with AI" button positioned between the default "Google Search" and "I'm Feeling Lucky" buttons?

10 May 2023 7,280 0 View

In 304H Stainless Steel which is the best etchant that can be used to reveal microstructure?

Kindly tell the electrolytic polishing reagent as well as chemical and electrolytic etchant for 304H austenitic steel.

19 March 2023 8,527 2 View

What are key practical techniques to do Climate Smart Agriculture (CSA)?

Looking for Climate-Smart agriculture (CSA) techniques which can be practically implemented in the existing agricultural fields i.e. on ground while farming.

26 January 2023 2,537 5 View

What is the difference between Taqman Universal master mix and Taqman gene expression mastermix ?

I have FACS sorted some different types of immune cells (Innate lymphoid cells, gamma delta T cells, Natural killer T cells and Natural killer cells). As the number of cells were less in number, I...

21 January 2023 3,778 0 View

Is it possible to retain the powdered/particle form of the hydride particles after coating it with polymer ?

Dear All, Can anyone please help in suggesting some relevant papers on "polymer-coated hydride particles" that give the product in a powdered form, not a composite? Thanks, Isha

17 January 2023 9,559 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,479 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,537 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,620 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 806 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,365 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,129 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,289 1 View