Daleep Arora

3 Questions 8 Answers 0 Followers

Questions related from Daleep Arora

In what time frame can Tunicamycin cause activation of caspase-1 or maturation of IL-1 beta in Bone marrow derived macrophages after priming with LPS?

I am trying to look at expression of mature IL-1 beta and caspase-1 activation at protein level. I am not very sure for how long I need to treat my cells with Tunicamycin or Thapsigargin to see an...

01 August 2017 8,418 4 View

Which are good antibodies for Endoplasmic Reticulum (ER) Stress pathway analysis to be used in INS- cells?

Hi Everyone I am looking for antibodies for ER pathways that works in INS-1 cells or any other cells. There are lots of paper out there but I want to know if anybody have tried these antibodies in...

19 January 2015 4,164 10 View

How to separate protein into oligomer and monomer form?

I am planning to separate the monomer and oligomer form of a protein using western blot. What kind of lysis buffer and loading buffer can be used for this purpose so as not to denature the protein?

08 December 2013 4,170 5 View