In western, can we strip membrane more than once? And any tips for the stripping solution?

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Kai Wong

Yes you can strip it for few times. Try the low pH stripping buffer to strip your membrane for 30 min. It works really well.

The recipe is: 1.5g glycine, 0.1g SDS, 1ml Tween 20, add 100ml di water, pH 2.2.

Good luck

Tatiana Sukhanova

Hi!

You can do untill 10 times but you should to follow at least one rule: 1st staining by "pale" antibodies toprotein of low stability and the last by the most stable one (beta-tubulin, for example).

Regards,

Tatiana