In western, can we strip membrane more than once? And any tips for the stripping solution?

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Jeff Zaki

I would say it is possible. You might as well try it out since you have less alternative options

Kai Wong

Yes you can strip it for few times. Try the low pH stripping buffer to strip your membrane for 30 min. It works really well.

The recipe is: 1.5g glycine, 0.1g SDS, 1ml Tween 20, add 100ml di water, pH 2.2.

Good luck

Tatiana Sukhanova

Hi!

You can do untill 10 times but you should to follow at least one rule: 1st staining by "pale" antibodies toprotein of low stability and the last by the most stable one (beta-tubulin, for example).

Regards,

Tatiana