Dear Norhaizan,

It was reported in review 1 (see below)  that mice that are genetically-engineered or carcinogen-induced to develop colon cancer rarely develop metastasis. In contrast, models using transplant colorectal cancers can exhibit high rates of metastasis and recapitulate some of the desired characteristics. In paper 2, the authors indicate that AOM rodent models can be used to study the metastasis

Review 1: Chin J Cancer. 2011 Jul; 30(7): 450–462.

doi:  10.5732/cjc.011.10041

PMCID: PMC4013420

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013420/

Mouse models of colorectal cancer

Yunguang Tong,1 Wancai Yang,2 and H. Phillip Koeffler1,3

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Abstract

Colorectal cancer is one of the most common malignancies in the world. Many mouse models have been developed to evaluate features of colorectal cancer in humans. These can be grouped into genetically-engineered, chemically-induced, and inoculated models. However, none recapitulates all of the characteristics of human colorectal cancer. It is critical to use a specific mouse model to address a particular research question. Here, we review commonly used mouse models for human colorectal cancer.

Paper 2: J Carcinog. 2011; 10: 9.

Published online 2011 Mar 24. doi:  10.4103/1477-3163.78279

PMCID: PMC3072657

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072657/

The AOM/DSS murine model for the study of colon carcinogenesis: From pathways to diagnosis and therapy studies

Mariangela De Robertis,1 Emanuela Massi,1 Maria Luana Poeta,2 Simone Carotti,3 Sergio Morini,3 Loredana Cecchetelli,4 Emanuela Signori,5 and Vito Michele Fazio1,6,*

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Abstract

Colorectal cancer (CRC) is a major health problem in industrialized countries. Although inflammation-linked carcinogenesis is a well accepted concept and is often observed within the gastrointestinal tract, the underlying mechanisms remain to be elucidated. Inflammation can indeed provide initiating and promoting stimuli and mediators, generating a tumour-prone microenvironment. Many murine models of sporadic and inflammation-related colon carcinogenesis have been developed in the last decade, including chemically induced CRC models, genetically engineered mouse models, and xenoplants. Among the chemically induced CRC models, the combination of a single hit of azoxymethane (AOM) with 1 week exposure to the inflammatory agent dextran sodium sulphate (DSS) in rodents has proven to dramatically shorten the latency time for induction of CRC and to rapidly recapitulate the aberrant crypt foci–adenoma–carcinoma sequence that occurs in human CRC. Because of its high reproducibility and potency, as well as the simple and affordable mode of application, the AOM/DSS has become an outstanding model for studying colon carcinogenesis and a powerful platform for chemopreventive intervention studies. In this article we highlight the histopathological and molecular features and describe the principal genetic and epigenetic alterations and inflammatory pathways involved in carcinogenesis in AOM/DSS–treated mice; we also present a general overview of recent experimental applications and preclinical testing of novel therapeutics in the AOM/DSS model.

Keywords: Animal model, chemical carcinogens, colorectal carcinogenesis, preclinical studies

Hoping this will be helpful,

Rafik

Dear Norhaizan,

It is depending on the type of studies you want to perform.

You can use more easy models like the B16F10 melanoma "pseudometastatic" lung model (see Mathieu et al., 2007) or an actual metastatic one, i.e. the subcutaneous P388 lymphoma model metastasizing to the liver (see Darro et al., 2005).

Best regards

Robert

Dear Robert

Thank you very much for your kind info.  Really appreciate it..