In vivo electroporation - which device are you using?

01 January 1970 0 6K Report

We have been using BTX AgilePulse for in vivo electroporation of mice for intramuscular delivery of DNA, but have some issues with the equipment.

Which device are you using? What is your experience? Other suggestions are appreciated.

Badges
Science topic

More Gry Persson's questions See All

Has GPS detected the ethrer bwind?

Abstract An illusion of undecidability of an ether wind was the basis for establishing the theory of special relativity (SRT). The reason to that mistake was a wrong interpretation of light...

08 February 2020 6,990 4 View

Bottle but not textile PET recycling - why not?

PET bottles are conveniently recycled into fibres by remelting and spinning. How come there is no fibre-to-fibre polyester recycling by re-melt-spinning? Is it due to degradation by i) hydrolysis...

24 June 2018 9,771 14 View

Anyone have experience working with chicken macrophage cell line HD-11? Which media to use? How to subcultivate them?

I have difficulties in growing HD11 cells. "In house" they have been cultivated in RPMI + 10% FBS, but I can see that others have used RPMI +2.5% chicken serum + 7.5% FBS or DMEM + 5 or 10%...

19 August 2013 1,294 5 View

Can I increase incubation of tissue samples in RLT buffer (Qiagen) for more efficient lysis?

I'm purifying total RNA from placental biopsies stored in RNAlater and -80'C. Biopsies (20 mg) are moved to RLT + 2-B-mercaptoEtOH for lysis using mortar and pestel or beads, before being...

01 January 1970 924 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View