Gry Persson

4 Questions 4 Answers 0 Followers

Questions related from Gry Persson

How do you isolate avian heterophils/neutrophils?

We have some difficulties in isolating the heterophils from chicken blood samples. Any experience and/or protocols?

09 April 2014 815 4 View

Anyone have experience working with chicken macrophage cell line HD-11? Which media to use? How to subcultivate them?

I have difficulties in growing HD11 cells. "In house" they have been cultivated in RPMI + 10% FBS, but I can see that others have used RPMI +2.5% chicken serum + 7.5% FBS or DMEM + 5 or 10%...

20 August 2013 1,374 5 View

Can I increase incubation of tissue samples in RLT buffer (Qiagen) for more efficient lysis?

I'm purifying total RNA from placental biopsies stored in RNAlater and -80'C. Biopsies (20 mg) are moved to RLT + 2-B-mercaptoEtOH for lysis using mortar and pestel or beads, before being...

01 January 1970 1,034 1 View

In vivo electroporation - which device are you using?

We have been using BTX AgilePulse for in vivo electroporation of mice for intramuscular delivery of DNA, but have some issues with the equipment. Which device are you using? What is your...

01 January 1970 6,270 0 View