I have been trying to evaluate the role of Kupffer cells (KCs) in my in vivo model and therefore I want to find a way to deplete this cell type. I have been looking in the literature and found some different treatments previously used such as gadolinium chloride and clodronate liposomes.
My current problem is that I dont want to deplete macrophages from other organs, specially macrophages from the spleen. Therefore I wanted to know how specific are those treatments to KCs and/or how to make sure that the splenic macrophages are functional.
I do not know if this will be helpful or not, but according to the Wikipedia page (see link below) Kupffer cells specifically express a protein called CRIg that binds inactive complement component C3. If there is a monoclonal antibody to this protein, you may be able to specifically deplete Kupffer cells.
https://en.wikipedia.org/wiki/Kupffer_cell
Your best option will be to use Clec4f–DTR mice. Then you can specifically deplete KCs.
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