In vitro transcribed RNA (IVT RNA) is a type of RNA that is synthesized in a laboratory setting using a process called in vitro transcription. This involves using a DNA template and enzymes to create a single-stranded RNA molecule in a test tube or other container. IVT RNA can be used for a variety of purposes, including research and gene expression studies.
Commercially purchased RNA refers to RNA that is obtained from a commercial supplier, rather than being synthesized in a laboratory. This RNA may be derived from a variety of sources, including cells, tissues, or organisms, and it may be purified and processed for use in research or other applications.
There are several key differences between IVT RNA and commercially purchased RNA:
Source: IVT RNA is synthesized in a laboratory using a DNA template and enzymes, while commercially purchased RNA is obtained from a commercial supplier.
Quality: Commercially purchased RNA is typically of higher quality than IVT RNA, as it has been purified and processed by the supplier.
Cost: Commercially purchased RNA is generally more expensive than IVT RNA, as it requires additional processing and handling by the supplier.
Versatility: IVT RNA can be synthesized to specific lengths and sequences, which allows for greater versatility in terms of its use and applications. Commercially purchased RNA is limited to the sequences and lengths that are available from the supplier.
Turnaround time: IVT RNA can typically be produced more quickly than commercially purchased RNA, as it does not need to be obtained from a supplier and does not require additional processing.