In the ORAC assay (Oxygen Radical Absorbance Capacity), at what concentration in μg/mL should I analyze my sample if it is a crude methanolic extract? Is it necessary to prepare a curve, or is a single concentration sufficient?"
For a crude methanolic extract in the ORAC assay, test multiple concentrations typically between 50 and 2000 μg/mL to ensure accurate measurement. Always prepare a Trolox standard curve to quantify antioxidant capacity as Trolox equivalents.