Can I use Chloroform, Acetone or Acetonitrile as Solvent in DPPH assay using UV Vis?

21 February 2024 1 5K Report

Hii.. I'm doing a riset with plant extract and I have got the pure compound from it.. I like to know the antioxidant activities from the crude extract and the compound. But, both of my samples don't soluble in methanol or ethanol.

Can I use Chloroform, acetone or Acetonitrile as the solvent instead of methanol since my samples are insoluble in methanol?

For information, I'm using alpha tocopherol as positive control.

Preety Rohilla

@Risna Ramadhani You can go with the same solvent which is used for the extraction of plant material.

Hope it"ll help.

Thanks

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