In terms of protein expression in the HEK cell 293 F type in the form of suspension, why the cell viability after transfection decreases so much?

Before transfection the viability is around 98% but exactly one day after I add the plasmid and PEI the viability of my cells decreases so much. I check the viability with the Trypan Blue 100...

14 September 2023 5,309 1 View