Dear Channa,

As yo mentioned that the numbervof reverse and forward primer is not equal, but it is not important. You can just pick em and me primers and use them. You can also use previous studies

Dear Dr Channa Siraj Ahmed,

Thank you for your question. Their are several markers can used for genetic diversity study i.e., Morphological and DNA markers i.e., RAPD, SSR, SNP, Ests and others

with bets regards

Khaled