In situ hybridization?

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What is the origin of the island theory in electronic governance?

exhaustively give its origin, relevance to electronic governance and how useful the theory will be to the government of Kenya. Give academic citations

06 March 2024 5,617 0 View

Quantification of double-positive cells using ImageJ software?

Dear all, I am working with melanoma cryosections from minipigs. My aim is to characterize tumor-infiltrating immune cells between different age groups, mainly by immunohistochemistry. I do...

29 January 2024 9,138 1 View

What are the future trends in AIS research?

بصفتك اكاديمي ومن خلال اطلاعك على البحوث والدراسات المتعلقة بنظم المعلومات المحاسبية ماهي الاتجاهات المستقبلية في ابحاث AIS

09 January 2024 4,753 0 View

Any idea about phase separation problems with commercial 25:24:1: Phenol: chloroform: isoamyl solution?

Hi, I am using a commercial 25:24:1: phenol: chloroform: isoamyl solution (which was bought less than 5 months ago), and I have two separate phases (two layers) now in the bottle even though there...

30 October 2023 7,689 0 View

Why the mean insert size in Sequencing is less than the DNA library size?

I have prepared DNA libraries; the fragment size is about 300 to 700 bp. In the sequencing results, I see the Mean insert size is 42bp.? The sequencing platform used was Novaseq PE150. DNA...

28 September 2023 9,398 1 View

Are there those who help to publish an article in the Scopus Journal. We can issue an article in cooperation?

Today, scopus has a hard time publishing articles in scientific journals, so we want to get help from experienced specialists. We think that this mihim will be considered to popularize Ilim. The...

28 September 2023 4,044 1 View

Do you have an idea about the concept of sentiment analysis ?

22 August 2023 4,607 3 View

Opinion in you reliability of the transaction via the Blockchain ?

opinion in you reliability of the transaction via the Blockchain ?

22 August 2023 1,927 4 View

Is geopolitics a bad science?

12 June 2023 8,831 0 View

Is sport a new actor in international relations?

12 June 2023 831 8 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View