Is there any free online plagiarism check software?

Hello everyone, I wish to check plagiarism for my article. Is there any authenticated free online tool for the same? Kindly advise.

05 June 2018 4,473 0 View

SiRNA dose administration to rats?

I am planning to inject siRNA locally to rats joint tissue. Till what dose of siRNA, I could administer to rats without causing any toxic effects. Can I adminster 20 microgram of siRNA in rats...

09 October 2017 2,012 1 View

What is an appropriate statistical tests for more than two groups?

Hello, I have more than two groups each containing the unequal number of samples. (group 1= 5 samples; Group 2, 3, 6=4 samples; Group 4,5=3 samples). I ran western blot of each sample. Now, I am...

06 July 2017 4,499 6 View

How to deliver siRNA in vivo (rats)?

10 November 2016 5,023 6 View

How cytokine stimulation can be done in primary cells?

07 August 2016 897 5 View

How can I avoid clump formation of cells during FACS (fluorescence activated cell sorting)?

Hi everyone, While doing FACS from human blood cells, I felt cells to be very sticky and hence they form clump which is very difficult to re-suspend in PBS. PBS, I use is calcium and magnesium...

05 June 2015 4,307 16 View

Why negative control wells show readings in Sandwich ELISA?

Hi everyone, While doing sandwich ELISA, every-time I face the problem of positive readings in negative control wells regardless of the sample type or protein targets against which primary...

04 May 2015 4,567 4 View

Any advice on western blot for ubiquitin?

10 November 2014 7,830 5 View

What is the loading control for tissue lysate and blood plasma?

What are the points that we have to keep in mind while selecting a suitable loading control for tissue lysate and body fluid such as blood plasma? Please help me.

10 November 2014 7,011 4 View

How do you do a western blot for ubiquitin?

I am interested in ubiquitin western blotting. I would like to know, if I use ubiquitin monoclonal antibody, will it give only a single band corresponding to it or will it be able to recognize...

10 November 2014 452 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View