I am planning to inject siRNA locally to rats joint tissue. Till what dose of siRNA, I could administer to rats without causing any toxic effects.
Can I adminster 20 microgram of siRNA in rats joint without having any toxic effects.
Please help!!
Hello Pooja.
That is a hard question to answer. However, you could roughly consider the local target tissue volume. This is the way that we determine dosage of our new test materials in the whole body. If you use 6ug/mL siRNA to transfect 70% confluent cells in a 60mm culture dish containing 6 mL medium, the siRNA dosage will be 1 ug siRNA/mL at final concentration in the medium volume. If the estimated vol of tissues in the joint is ~2 mL (cm3), the calculated siRNA dosage would be 1ug/1 mL at the same ratio of 6 ug/6 mL as in cell culture. Therefore, this simple method of estimation was used in our dosage of test materials in MPK (mg test materials per kg body weight) by just considering the body weight as volume although the body consists of about 60% water. The only caution we took was warm solution, and very slow injection with gentle massaging the tissues to allow even diffusion and relieve pain.
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