Ajeet Singh

8 Questions 36 Answers 0 Followers

Questions related from Ajeet Singh

Specific heat capacity and Enthalpy calculation from Heat flow value in DSC curve.

I want to calculate specific heat of my aggregation reaction and carried out a DSC with following parameters. 1.the sample mass (7.8mg) 2. heating rate (0.5 deg/min) 3. range (30 to 90c) output...

12 December 2015 1,007 20 View

How to make a protein soluble?

My expressed protein is being collected in the pellet fraction and I have used different strategies for making it soluble but still its coming in the pellet. I have used strategies like...

02 February 2014 9,814 32 View

What methods can I use to induce a Thermus thermophilus protein?

I am working on thermus thermophilus protein which is not being expressed. I have tried that particular protein induction in a different time scale (12-37 degree), different IPTG concentration...

02 February 2014 2,564 6 View

Shifting of protein band in SDS PAGE - can anyone help?

I cloned my gene of interest in pET28a vector, my protein molecular weight is 24 kDa but its coming at 27 kDa, why is there such shifting in SDS PAGE?

10 October 2013 5,492 7 View

What to do when protein is getting precipitated during dialysis?

I purified my recombinant His tag protein in Tris, pH 7.5 (pI of protein 8.9) using 400mM Imidazole conc. in elution buffer. I dialysed my protein for removing imidazole but it is being...

10 October 2013 3,640 22 View

How does one decide which solvent system I should run my protein sample in HPLC?

I have purified my protein which MW is 27kDa for crystallization purpose but some impurities are there. So I want to do HPLC for further purification but I am not having exposure for HPLC work, I...

10 October 2013 7,132 4 View

Non specific amplification in PCR?

I am getting non specific PCR, I have tried with increase temperature and decreasing primer concentration but still non specificity is there? What parameters should I consider now?

10 October 2013 1,922 13 View

Which buffer is the better choice for membrane protein purification.

I have tried to purify membrane protein in HEPES buffer but still there are some other buffers there. Which buffer and other biochemical parameters should be followed for better protein induction...

01 January 2013 9,102 6 View