Hello everyone,
I wish to check plagiarism for my article. Is there any authenticated free online tool for the same?
Kindly advise.
I am planning to inject siRNA locally to rats joint tissue. Till what dose of siRNA, I could administer to rats without causing any toxic effects. Can I adminster 20 microgram of siRNA in rats...
09 October 2017 2,106 1 View
Hello, I have more than two groups each containing the unequal number of samples. (group 1= 5 samples; Group 2, 3, 6=4 samples; Group 4,5=3 samples). I ran western blot of each sample. Now, I am...
06 July 2017 4,786 6 View
I want to perform an siRNA mediated silencing of a gene in rat. I am new to this experiment. Please advice an effective protocol for the same and precautions that i need to keep in mind. Thanks
10 November 2016 5,108 6 View
Hii everyone, I am interested in treating primary cells with some cytokines. I found these cells do not grow properly in serum free media. However, literature suggests the treatment should be...
07 August 2016 973 5 View
Hi everyone, While doing FACS from human blood cells, I felt cells to be very sticky and hence they form clump which is very difficult to re-suspend in PBS. PBS, I use is calcium and magnesium...
05 June 2015 4,672 16 View
Hi everyone, While doing sandwich ELISA, every-time I face the problem of positive readings in negative control wells regardless of the sample type or protein targets against which primary...
04 May 2015 4,755 4 View
What are the points that we have to keep in mind while selecting a suitable loading control for tissue lysate and body fluid such as blood plasma? Please help me.
10 November 2014 7,099 4 View
I wish to perform western blotting for ubiquitin. In my earlier attempt I could not see the band corresponding to 8.5 kda in a 12% SDS gel. Please help me.
10 November 2014 7,983 5 View
I am interested in ubiquitin western blotting. I would like to know, if I use ubiquitin monoclonal antibody, will it give only a single band corresponding to it or will it be able to recognize...
10 November 2014 585 3 View
During my western blot my protein gives band nearly between 42- 52 KDa. I want to use beta actin or beta tubulin as my loading control. But, unfortunately it also gives band in the same region....
10 November 2014 867 4 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...
05 August 2024 5,311 0 View
for eg. transition shock scale
01 August 2024 5,998 0 View
Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.
01 August 2024 10,046 1 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
I performed mmpbsa of protein-protein complex simulation on gromacs. My binding free energy is +166781. This complex is stable when experimentally produced in wet lab. In index.ndx I grouped...
19 July 2024 4,120 5 View
I would like to use biofilmQ to analyse confocal laser image, how can do?
16 July 2024 7,628 2 View
Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...
14 July 2024 7,475 1 View