SiRNA dose administration to rats?

I am planning to inject siRNA locally to rats joint tissue. Till what dose of siRNA, I could administer to rats without causing any toxic effects. Can I adminster 20 microgram of siRNA in rats...

09 October 2017 2,012 1 View

What is an appropriate statistical tests for more than two groups?

Hello, I have more than two groups each containing the unequal number of samples. (group 1= 5 samples; Group 2, 3, 6=4 samples; Group 4,5=3 samples). I ran western blot of each sample. Now, I am...

06 July 2017 4,499 6 View

How to deliver siRNA in vivo (rats)?

10 November 2016 5,023 6 View

How cytokine stimulation can be done in primary cells?

07 August 2016 897 5 View

How can I avoid clump formation of cells during FACS (fluorescence activated cell sorting)?

Hi everyone, While doing FACS from human blood cells, I felt cells to be very sticky and hence they form clump which is very difficult to re-suspend in PBS. PBS, I use is calcium and magnesium...

05 June 2015 4,307 16 View

Why negative control wells show readings in Sandwich ELISA?

Hi everyone, While doing sandwich ELISA, every-time I face the problem of positive readings in negative control wells regardless of the sample type or protein targets against which primary...

04 May 2015 4,567 4 View

Any advice on western blot for ubiquitin?

10 November 2014 7,830 5 View

What is the loading control for tissue lysate and blood plasma?

What are the points that we have to keep in mind while selecting a suitable loading control for tissue lysate and body fluid such as blood plasma? Please help me.

10 November 2014 7,011 4 View

How do you do a western blot for ubiquitin?

I am interested in ubiquitin western blotting. I would like to know, if I use ubiquitin monoclonal antibody, will it give only a single band corresponding to it or will it be able to recognize...

10 November 2014 452 3 View

What can I do when the band for loading control coincides with my protein band in Western blots?

During my western blot my protein gives band nearly between 42- 52 KDa. I want to use beta actin or beta tubulin as my loading control. But, unfortunately it also gives band in the same region....

10 November 2014 777 4 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Can anyone recommend which is the best free questionnaire software please?

Hello, I am looking to send out links to an online survey. My previous research project used a paid for survey software. I am now looking for something that's free but straight forward to...

19 February 2021 5,641 3 View