15 Questions 7 Answers 0 Followers
Questions related from Pooja Kumari
Hello everyone, I wish to check plagiarism for my article. Is there any authenticated free online tool for the same? Kindly advise.
06 June 2018 4,514 0 View
I am planning to inject siRNA locally to rats joint tissue. Till what dose of siRNA, I could administer to rats without causing any toxic effects. Can I adminster 20 microgram of siRNA in rats...
10 October 2017 2,069 1 View
Hello, I have more than two groups each containing the unequal number of samples. (group 1= 5 samples; Group 2, 3, 6=4 samples; Group 4,5=3 samples). I ran western blot of each sample. Now, I am...
07 July 2017 4,581 6 View
I want to perform an siRNA mediated silencing of a gene in rat. I am new to this experiment. Please advice an effective protocol for the same and precautions that i need to keep in mind. Thanks
11 November 2016 5,069 6 View
Hii everyone, I am interested in treating primary cells with some cytokines. I found these cells do not grow properly in serum free media. However, literature suggests the treatment should be...
08 August 2016 936 5 View
Hi everyone, While doing FACS from human blood cells, I felt cells to be very sticky and hence they form clump which is very difficult to re-suspend in PBS. PBS, I use is calcium and magnesium...
06 June 2015 4,466 16 View
Hi everyone, While doing sandwich ELISA, every-time I face the problem of positive readings in negative control wells regardless of the sample type or protein targets against which primary...
05 May 2015 4,666 4 View
During my western blot my protein gives band nearly between 42- 52 KDa. I want to use beta actin or beta tubulin as my loading control. But, unfortunately it also gives band in the same region....
11 November 2014 826 4 View
I am interested in ubiquitin western blotting. I would like to know, if I use ubiquitin monoclonal antibody, will it give only a single band corresponding to it or will it be able to recognize...
11 November 2014 530 3 View
What are the points that we have to keep in mind while selecting a suitable loading control for tissue lysate and body fluid such as blood plasma? Please help me.
11 November 2014 7,058 4 View
I wish to perform western blotting for ubiquitin. In my earlier attempt I could not see the band corresponding to 8.5 kda in a 12% SDS gel. Please help me.
11 November 2014 7,932 5 View
See above
08 August 2014 1,794 3 View
Is ELISA (Sandwich ELISA) suitable for expression validation of an intracellular protein from tissue lysate containing lysis buffer?
08 August 2014 8,743 2 View
I am searching for some reliable Bioinformatic tools that could help me in predicting protein-protein interaction (PPI) of a selected protein or better it will be if I could study interactomics of...
08 August 2014 1,303 4 View
During Immunohistochemistry (IHC) image analysis for tissue sections, I am facing the problem with negative control tissue (i.e. without primary antibody), as it is also showing positive staining...
08 August 2014 163 15 View
