I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Does anyone ever solved this following Matlab error : "Error using imaqdevice/start (line 95) pointgrey: Failed to stop the camera acquisition." ?

I use Matlab 2014b to capture some images with a PointGrey camera connected with USB3 port. I initialize the camera in a function which basically execute these followed lines : vid =...

22 February 2021 6,386 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

What are the main conditions suited for synthesizing a PVDF thin film by solvent casting method?

I have been trying to synthesize PVDF thin film by solvent casting method. I started by using 2:3 DMSO: acetone as solvents. The solution was stirred for 3hrs at 60 deg and was dried at 90 degrees...

09 February 2021 8,850 3 View

Wave velocity and rock density paradox?

Practically it is known that seismic velocity increases as rock density increases, why then does the wave velocity equation involving elastic moduli and density indicate that velocity is inversely...

09 February 2021 1,843 3 View

What is the magnitude of yearly rhizopdeposition in tropical forests?

Hi all, I am trying to find reference values on annual soil rhizodeposition rates for different kinds of forests, with a particular interest for tropical rainforests. Do we have an idea of the...

26 January 2021 4,278 4 View

Recipe for old qiagen DNA extraction buffer AP2?

Hi there, I'm looking for the recipe for a buffer no longer made by qiagen, but that i need for a procedure i'd like to carry out. I'd like to follow the extraction procedure laid out in the...

17 January 2021 8,496 1 View

Will an electromagnet core be as efficient if it is made of several pieces?

If you had a design for an electromagnet core that required it to be assembled out of several parts would it still produce as strong a magnetic field as the same core but made out of a single...

17 January 2021 5,821 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Can Dynasore inhibit Caveolae-mediated endocytosis?

HI, I'm studying endocytosis pathway of certain particles, using Dynasore and MbCD endocytosis inhibitors. My question is, that whether dynasore could inhibit both clathrin-ME and...

08 May 2020 9,372 1 View

What is the average half-life of a typical membrane protein?

Just a generic question, what is the average half-life of a typical membrane protein? If you have a review off hand that could be sent to me, that would be greatly appreciated.

03 April 2019 197 3 View

Looking for a protocol for 4 degree incubation for cells?

I want to decrease endocytosis in my cells and therefore want to incubate them at 4 degrees. I was wondering how long can cells stay at this temperature ? If i wanna use some dye like DAPI will it...

28 November 2018 4,158 3 View

Looking for a protocol for 4 degree incubation for cells?

I want to decrease endocytosis in my cells and therefore want to incubate them at 4 degrees. I was wondering how long can cells stay at this temperature ? If i wanna use some dye like DAPI will it...

10 November 2018 3,651 3 View

What does it mean when Nanoparticle uptake is unchanged for cells treated with dynasore and reduced in case of chlorpromazine and genistein?

When I am treating cells with endocytosis inhibitors Dynasore, chlorpromazine and genistein for 1 hour and then exposing them to nanoparticles for a period of 1 hours, the internalization of my...

09 October 2018 1,108 0 View

Inhibitors of EGFR internalization?

In our lab, to block internalization and endocytosis of EGFR, an inhibitor solution of 2 mM of sodium fluoride (NaF), 10 mM of sodium azide (NaN3) and 5 mM of 2-deoxy-D-glucose is used. Someone...

08 October 2018 4,646 3 View

Does reduced fluorescence in lysosomes using the DQ-BSA assay mean reduced lysosome activity or reduced endocytosis?

We have used the DQ-BSA assay to examine lysosome activity. We co-localize lysotracker with the DQ-BSA fluorescent signal. In one particular protein knockdown we see normal lysotracker...

07 August 2018 4,497 0 View

How to look hany receptors have been endocytosised by western blot?

I am trying to do DDR1 receptor endocytosis test. I use collageI coated fask to activate DDR1 and want to know if DDR1 will be phophorylated and be endocytosed in to the cell. how can I exclude...

07 August 2018 1,003 2 View

Antibody internalization after fixation with paraformaldehyde in cell ELISA assay?

Hi, An antibody specific to the extracellular domain of HER2 receptor was produced. It is used as both target protein and primary antibody on SKBR3 cell ELISA assay in order to confirm its binding...

05 June 2018 9,763 2 View