I am interesting in dumping protein cargo during endocytosis (pH 5-6) and interested to know if other researchers work with or have come across such a linker? Thanks for any advice or insights.
… with my present female lover … she and I spend anywhere from two hours to six hours in caressing, touching, cuddling, hugging, lip kissing, deep kissing and intimate conversation before,...
11 August 2024 4,521 0 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
Your partner’s not there to service you, it’s not their job to keep you sexually satisfied. You’re together because you love each other and want to make each other happy. Constantly hassling them...
08 August 2024 1,491 0 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
What is the method for analytically calculating the wear and service life of polymer-polymer sliding pairs?
04 August 2024 1,078 1 View
Women often complain that their husbands never touch them unless they want sex. (Michele Weiner-Davis)
02 August 2024 7,778 2 View
Women need to feel a degree of sexual intimacy before sex becomes desirable… For women, intimacy sometimes results in sex; for men, sex sometimes results in intimacy. (Marina Muratore)
31 July 2024 8,860 0 View
Women associate affection with love. … Men associate affection much more directly with sex. … Men see affection of any kind as a sexual invitation. Many women find this bewildering. (Kramer &...
30 July 2024 9,498 2 View
If the pairs are similar, will it reduce the adhesion due to electric charges?
29 July 2024 3,185 2 View
Women also often find it easier to fantasise when self-pleasuring than in sex with a partner. The immediacy of someone else’s needs actually inhibits the expression and satisfaction of their own....
26 July 2024 8,351 2 View
I want to asses percentage of dead cells taken up by macrophages. If I stain dead cells with one of Thermofishers LIVE/DEAD stains, will this be transferred to macrophages that take them up by...
20 December 2023 1,055 0 View
"Huh7 cells were incubated for 6 h with DEN-1 sNS1 (10 ug/ml) or PBS, followed by 24 h or 48 h of chase (6 h/24 h and 6 h/48 h, respectively)." This statement is from the paper titled "The...
04 October 2023 5,082 1 View
Hey all, I just needed a clarification on why cytochalasin D is added for assessing endocytic capacity of dendritic cells? I have plans to use FITC-conjugated dextran to assess DC endocytosis, and...
03 July 2021 4,704 3 View
¿Why are both bands present in cells cultured in complete medium recovered after shorter time in culture whereas the upper band seems predominant at longer times? ¿and why the behaviour of the...
14 April 2021 6,044 1 View
Measles virus attaches with protein H to the surface of cell, once this happens it gives signal to the fusion protein F, which becomes active and initiates virus entry into the cell by membrane...
27 March 2021 7,961 2 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,771 2 View
Hello everyone, Coming from a background of materials and X-ray science, I have little knowledge about endocytosis and cytotoxicity. I wonder if I add gold nanoparticles (AuNPs, citrate modified,...
09 August 2020 1,186 2 View
Does the COVID19 virus integrate into human cells by injecting its RNA or does it endocytosis?
05 August 2020 3,314 4 View
HI, I'm studying endocytosis pathway of certain particles, using Dynasore and MbCD endocytosis inhibitors. My question is, that whether dynasore could inhibit both clathrin-ME and...
08 May 2020 9,568 1 View
Just a generic question, what is the average half-life of a typical membrane protein? If you have a review off hand that could be sent to me, that would be greatly appreciated.
03 April 2019 384 3 View