thank you for posting this interesting technical question. There are various protocols for this test available on the internet. For example, please check out the following instructive YouTube videos:
Phenol-Sulfuric Acid Method for Carbohydrates
https://www.youtube.com/watch?v=So6O62iC8YA
and
Estimation Of Total Carbohydrates By Phenol Sulphuric Acid Method |Total carbohydrate Determination
https://www.youtube.com/watch?v=HVBBHFHjZ5I
I hope this helps. Good luck with your work and best wishes, Frank Edelmann