In PCR, why do the salts differ for particular polymerase enzymes like Mgcl2 for Taq and MgSo4 for Kod polymerase?

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Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

Can anyone suggest some monoembryonic rootstock of mango with abiotic or biotic resistance?

17 April 2024 1,568 1 View

How to get a smoother DOS plot in VASP?

Hello All! I am hereby attaching the DOS plot for my system. As you can see, it has too many spikes., however I want a smoother plot. I have used ISMEAR = -5 and NEDOS = 3000. Do I need to make...

19 March 2024 4,457 4 View

Why adonitol or ribitol is used as a internal standard in GC-MS untargeted metabolomics of plant?

26 December 2023 6,148 0 View

Which is the best medium to store extracted deciduous/primary teeth for research purpose over a long period of time (6-12months or more)?

Many in-vitro studies are done on primary teeth, especially to study their internal structure. We have to store a sufficient number of teeth before we start our study. Which solution would be the...

21 December 2023 5,693 4 View

Which Indices are suitable to assess the gingival health in primary and mixed dentition for pediatric population?

To assess the gingival health in pediatric population, what indices are recommended?

07 August 2023 9,704 7 View

What are index teeth in Gingival Index by Loe and Silness (1963) for assessment in Primary and Mixed dentition??

The Gingival Index by Loe and Sillness (1963) is widely being used in epidemiological studies to assess the Gingival health. The modified scale assesses 6 index teeth in the permanent dentition to...

07 August 2023 3,713 3 View

What are key points when performing Arabidopis leaf tissue staining with FM4-64 dye?

I want to study endocytic pathway for a specific plant protein using FM4-64 dye and leaf tissue from A. thaliana transgenic plants. Most of the studies were performed using only root tissues. Have...

01 May 2023 5,306 0 View

Has anyone done a PES Scan in gaussian using Centroid defined by GIC?

I am trying to do a potential energy scan of a BTA (N,N',N''-trismethyl-benzene-1,3,5-tricarboxamide) dimer. I defined the coordinates of the COM (I choose 6 carbons of benzene ring for this)...

30 April 2023 557 0 View

Does anyone check the expression of GABARAP protein in GBM cell line ( U87MG) ?

My westerns haven't worked for the past three to four months, which is an issue. I attempted using two different antibodies from two different companies, but I got no blots. I load 50 to 100 ug of...

22 April 2023 1,331 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View