Megha Achar

4 Questions 10 Answers 0 Followers

Questions related from Megha Achar

I had done PCR standardization to amplify my gene of interest of 1.5kb from cDNA. When I repeated the same protocol, no amplification was seen. Why?

I had done PCR standardization using cDNA as template. I did too many pcrs and finally standardized to amplify the gene of interest of 1.5kb from cDNA. I amplified 2 different genes but of same...

17 November 2014 2,051 7 View

I did cloning for phusion protein. I did westerns and getting a band of interest along with a band with low molecular weight, why?

I had cloned my gene of interest in to a vector, which has fluorescent protein tag. I did restriction digestion and did insert release of 1.4kb and fragment release having both gene of interest...

15 July 2014 771 7 View

Can I use a combination of acids like Hcl and H2so4 / Hno3 in adjusting the pH of Tris buffer saline?

Tris buffer saline of pH-7.4. As in case of emergency like one of the above mentioned acid gets over while adjusting the pH, than rest of the pH could be adjusted using other alternative acid. Is...

21 July 2013 7,036 2 View

In PCR, why do the salts differ for particular polymerase enzymes like Mgcl2 for Taq and MgSo4 for Kod polymerase?

However both salts have Mg ions.

01 June 2013 4,265 2 View