In order to prepare linearized DNA that I will electroporate into mouse ES cells, should I do chloroform&alcohol precipitation or which other method?

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Could anyone recommend a stable high transfection efficiency mouse cell line that I could use?

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Does anyone have some experience/useful advice for isolating single clones?

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Could anyone recommend some methods (e.g. staining) for testing if cultured MEF cells are indeed MEF cells?

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How to increase a model accuracy ?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Which cell line should we use to perform biological dosimetry experiments?

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Why is the efficiency of viral mRNA electroporation in PBMC low?

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Has anyone used the Illumina ITS1 primer sets?

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Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

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Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

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What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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Wolfgang Hennig

Phenol/chloroform ist still one of the most effective (and cheapest) DNA isoklation methods. However you should afterwards use another chloroform extraction to prevent contamination with remaining phenol!

Michael Tung

In my experience, column purifications like the mini prep by QIAGEN have residues left over post clean up that could perturb the integrity of your dna sample. I've always preferred to do ethanol precipitation due to the high amount of purity I get for a reasonable end yield.