Dora Tarlungeanu

4 Questions 3 Answers 0 Followers

Questions related from Dora Tarlungeanu

In order to prepare linearized DNA that I will electroporate into mouse ES cells, should I do chloroform&alcohol precipitation or which other method?

I have been reading that this chloroform/phenol extraction and alcohol precipitation is a usual method for DNA extraction prior to ES cell electroporation but I was wondering if anyone knows why...

02 February 2015 1,853 2 View

Does anyone have some experience/useful advice for isolating single clones?

I am currently trying to isolate single clones, post-transfection with a GFP expressing plasmid so that I can evaluate the efficiency of the CRISPR technique. I tried single clone isolation by...

05 May 2014 8,616 16 View

Could anyone recommend a stable high transfection efficiency mouse cell line that I could use?

I've been trying to transfect MEF cells using several methods and I did not get a very high efficiency (we have the Nucleofection kit too but there are many constructs that I need to transfect and...

05 May 2014 10,024 10 View

Could anyone recommend some methods (e.g. staining) for testing if cultured MEF cells are indeed MEF cells?

I have been culturing MEF cells and tried to use different transfection methods to transfect them and they show very low efficiency and we would like to make sure that they are indeed MEFs so that...

04 April 2014 2,889 4 View