There is a difference between extracting polyphenols only for in vitro tests and in vivo or animal ones. For extracting and evaluation of the content only, we use generally methanol or ethanol at 80%, 70% and 50% with water (the three most polar solvent). For animal tests, it's better to use only water maceration by heating as decoction or infusion. The evaporation of solvent for in vivo tests is obligatory, otherwise the activity could be due to solvent, not to your plant.

Methanol/ethanol can extract most compounds from macerated products

Methanol 80% is the best solvent for the extraction of different polyphenols

Hello, Abir,

Methanol and ethanol are good solvents. You may find this article interesting for an optimized protocol: https://www.ncbi.nlm.nih.gov/pubmed/26471536.

Hope this helps.

Best,

Arizaldo

If you can use the microwave, it's the best one (house microwave) and you can use a water as solvent in order to testing the extract without any effect on your animal model.

For maceration, ethanol, water, HCL, and also a mixture of water with ethanol and HCL can be used.

Hello, from my experience, the methanol (80%) is the solvent you can use. Since you work on animals, you can use water ou ethanol which are without effect (toxicity) on your animals.

you can consult this work:

http://www.ifrj.upm.edu.my/21%20(03)%202014/31%20IFRJ%2021%20(03)%202014%20Benmeziame%20357.pdf

I recommend using ethanol 80% because it is less toxic.

If you want to get good extraction yield you should choose the solvent based on the polarity of target compound(s) that you are going to extract. You can get different degree of polarity by mixing different solvents such as water and ethanol at different mixing ratio.

Ethanol and methanol are good solvents for maceration.

Ethanol and h2o are good solvents for maceration.

Article Optimized microwave-assisted extraction of total phenolics (...

There is a difference between extracting polyphenols only for in vitro tests and in vivo or animal ones. For extracting and evaluation of the content only, we use generally methanol or ethanol at 80%, 70% and 50% with water (the three most polar solvent). For animal tests, it's better to use only water maceration by heating as decoction or infusion. The evaporation of solvent for in vivo tests is obligatory, otherwise the activity could be due to solvent, not to your plant.

wich solvant is better for analywing the antimicrobial activity of plant extracts