I am doing the egg albumin denaturation test to evaluate the anti-inflammatory activity of my SCE. After keeping the reaction mixtures in the water bath, the egg albumin gets denatured while forming clumps. These clumps precipitate. Do I have to break down the clumps and make a homogenize mixture before measuring using a spectrophotometer? If so what is the most suitable way to do that? Or do I have to take the readings with the clumps which are varying in size and that may cause significant variation in the readings?
Do you want final results or also interested in knowing the size of clumps being formed during the precipitation? I think it will be better that you take absorbance before the denaturation, followed by denaturation. Further, you have to centrifuge the precipitate and take absorbance of supernatant, and by comparing both the absorbance you can have the idea of amount of albumin being denatured during your experiment.
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays