Due to the prevailing condition, it is unable to conduct the extraction procedure(Rotary evaporation). The extract is in DCM and methanol mixture for nearly 2 months. Will the compounds degrade over time? Is it better to start all over?
Dear Dr Janaka Kuruppuarachchi ,
Best way to heading with ultrasound assisted extraction, if it is available.
Dear Dr Janaka Kuruppuarachchi ,
Best way to heading with ultrasound assisted extraction, if it is available.
We usually follow a literature extraction protocol based on which we will receive the most bioactive extract. The stability of the bioactivity of the extract is clearly dependent on the storage conditions. Its has observed that the bioactivity of the extract started to decrease, probably from oxidation processes caused by light, temperature and oxygen exposure.
Please think to change your solvents with ethanol because are toxic and can not be completely recycled and there are always losses. Like mr. Samanarathna already has proposed you have to consider to use non-conventional extraction technics as ultrasound-assisted and microwave-assisted extractions if there is such a possibility.
Hello,
To my knowledge, the only way to keep bioactive molecules for long periods is storage at -80 ° C. Most of the molecules extracted have antioxidant activity due to their ability to trap free electrons or even free radicals. Therefore, they are reactive which will inevitably lead to their degradation (especially in the presence of light and oxygen).
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