If the only difference is library size, read depth should not be affected.
Hi George, thanks for ur reply.
I did not understand why I got higher reads in some of the samples.
My pooled library had 12 libraries, 8 of them were almost of equal size (450-600bp) and 4 of them were larger in size (604bp, 812bp, 1023bp, 1323bp) and in the indexing QC result, % Reads identified(PF) vs index number these 4 had high reads (upto 25%) while other 8 had similar lower reads.
What is the reason? And is it necessary to get library size of around 500bp for a good sequencing?
What will you suggest?
How did you pool your 12 libraries? and I also suggest adjusting your fragment conditions. In my opinion, the DNA fragments are about 200-300bp, so the library are about 300-400bp. General speaking, library with smaller size have higher reads (equal mole).
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